2.11 Describe experiments to investigate how enzyme activity can be affected by changes in temperature.

As you should know, the test for starch is to put iodine on a sample, and it will turn blue black if starch is present (or it may turn another color but the exam answer is blue black). To investigate how temperature affects the enzyme rate of reaction, you could do the following experiment:

• Put x amount of starch in a test tube (eg 10 cm3) 
• Add amylase to it
• Put the mixture into a waterbath at temperature y
• On tiles with little dimples (WHAT IS THAT CALLED?!), use a dropper/pipette to put a small sample of the mix (of what is in the test tube) onto one of the 'dimples' and add iodine to it. Do this every thirty seconds.
• Time how long it takes for the samples to stop turning blue black
• Repeat everything again, but at at different temperature. An example would be do do the experiment at 15ºC, 25ºC, 35ºC, 45ºC and 55ºC (in other words, you change temperature y)
• Plot a graph

Figure 1: What your graph would look like (something similar)