Wednesday, February 22, 2017

5.19 Describe the stages in the production of cloned mammals involving the introduction of a diploid nucleus from a mature cell into an enucleated egg cell, illustrated by Dolly the sheep


The stages are as follows:
  1. Remove a donor (diploid) cell with the full genetic information (contained in the nucleus)
  2. A cell must be obtained, but one with the capacity to divide. Thus, an egg cell is taken from a sheep.
  3. The egg cell nucleus is removed (it is enucleated)
  4. Desired DNA is placed into the egg cell. They are fused together with an electric shock. 
  5. This newly combined cell divides by mitosis to produce a sheep embryo.
  6. The embryo is placed into the womb of another sheep (the surrogate mother)
  7. As the cells continue to divide, it will grow into a fetus and eventually be born.
The cloned sheep will have the DNA of the cell donor.

Source: http://media1.shmoop.com/images/biology/biobook_biotechnology_graphik_18.png




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